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Lasers and the EyePDF-file
General Laser Safety Guidelines
Optical Tweezers Safety Precautions
Laser Safety Classification
Laser Safety Links

Example for a
Laser Safety Calculation



Lasers and the Eye
The unprotected human eye is extremely sensitive to laser radiation and can be permanently damaged from direct or reflected beams. The site of ocular damage for any given laser depends upon ist output wavelength. Lasers in the visible (VIS) and near infrared (NIR) range of the spectrum can cause retinal injury, since the cornea and the lens of the eye are transparent to these wavelengths. Furthermore the lens of the eye focusses the laser beam down to a very small spot of the retina, increasing the energy density in the resulting spot tremendously. Even a low power laser (with a few milliwatts) can cause a burn on the retina - especially in darkend space, where no other light sources are present, and the eye is focussed to infinity. Wavelenghts outside this region (UV, IR) are absorbed by the anterior segment of the eye, causing damage to the cornea and/or to the lens. The extent of ocular damage is determined by laser irradiance, exposure duration and beam size. The eye has a self defense mechanism, the blink or aversion response. This may defend the eye from damage where very low power and visible lasers are involved, but can not protect the eye where higher power or NIR lasers are concerned. By the time the eye reacts, the damage is already done.

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General Laser Safety Guidelines

-> Never look into the beam of any laser. How bright a beam appears has no bearing on the power level. It could emit any wavelength at any power. IR laser are invisible and even more dangerous, because your blink and aversion reflexes don't work, since you can't see the beam.

-> Specular reflections may be just as dangerous as the raw beam.

-> Always wear a proper set of eye goggles, especially when working with lasers rated for Class IIIb and Class IV. Take care you have the specific protection depending on your laser wavelength and power. The worst thing is to think you are protected when you are not.

-> Always block all beams you don't use. Terminate the laser beam with a light absorbing material or diffuse screen.

-> Instruct anyone else, who is working insight the room to the hazards of laser light, and make sure, they understand all.

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Optical Tweezers Safety Precautions

Lasers used in optical tweezers have potential to damage the retina. In case of invisible infrared lasers special care must be taken, because injury due to exposure may be initially undetected until thermal damage has occured. The retina lacks of pain sensory nerves and, since the radiation is invisible, the aversion response of the eye does of course not work. Special care must be taken at the eyepieces of the microscope. The simplest method is to remove the eyepieces and close the holes of the microscope head. If the eyepieces are used, proper filters must be installed and carefully tested. Be sure that they are installed permanently (into the head!) and can not be accitendally removed. Be careful when adjusting your optics. Always do your alignments using the minimum laser power possible. Check for strays every time you realign, also at full operating power. Laser beams should always propagate in a plain parallel to the optical board. If the laser is on, do not bring your eyes to this plain. Fasten your mirrors, lenses and other optical devices tide, so they can not move by chance, generating dangerous beams. Always wear laser safety googles until you turn off the laser. Be aware, that your neck and head are not protected.

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Laser Safety Classifications

All lasers are classified by the manufactorer and labelled with the appropriate warning labels.

-> Class I Lasers:
Lasers that are not hazardous for continuous viewing, or are designed in such a way that prevent human access to laser radiation [P<0,39µW].

-> Class II Lasers:
Lasers emitting visible light which, because of human aversion response, do not normally present a hazard. Viewing directly over an extented period of time would cause injuries [P<1mW].

-> Class IIIa Lasers:
Lasers that normally would not cause injury to the eyeif viewed momentarily but would present a hazard if viewed using collection optics [P<5mW].

-> Class IIIb Lasers:
Lasers that present an eye or skin hazard for both, direct beam and specular relections. Viewing diffuse reflection does not produce hazards, except at close proximity [P<500mW].

-> Class IV Lasers:
Lasers that present an eye hazard from direct, specular and diffuse reflections. Furthermore, those lasers may produce fire hazards and skin burns.

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Laser Safety Links

Laser Institute of America
Sam's Laser FAQ - Laser Safety:
a comprehensive page, concerning a variety of topics around laser safety
University of Waterloo - Laser Safety Manual
Institute of Laser Medicine (German)
Heinrich Heine Universität Düsseldorf, Germany;

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last modified: January 5, 2001
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